FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2765828
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10550.059.7215465
TCCCGTC6400.059.6088079
CTATAGG13050.059.009676
CGCTAGC2200.058.869466
TGATAGG29200.058.618816
CAGATAG10750.058.6104475
GATAGGA33950.057.9386377
GATAGGT18500.057.8923847
GGATAGT20900.057.6133546
GGATAGG18850.057.5653766
CCCGTCC7500.056.9327910
CCGATAC1850.056.762375
CGCGATA5650.056.3772624
CTAGGAC9000.055.9994668
GCGATAA10450.055.2686235
CACGATA6400.055.239844
GGGATAG16550.055.2018365
CGCTACA3300.055.156976
GATAGGG18450.055.0140237
CGCTATA7850.054.846194
Produced by FastQC (version 0.11.5)