FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118039
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG7150.061.6789477
CTGATAG17450.060.7771265
GATAGGA23450.059.851357
CCTGATA6700.059.5556564
TGATAGG21700.058.8743446
GCTGATA16650.058.0213474
CGATATG9100.057.6956186
CTAGGAC5050.057.523988
CGGGATA6450.057.522684
ATAGGTC5300.056.7916838
ATAGTCG3700.056.755998
CCGATAA7450.056.379075
TATACGG4600.056.3049167
GGATAGT12700.056.2236986
GTATAGG12550.056.058996
CGATAAA13300.055.792676
GATAGTC9100.055.7698027
CGTATAG7300.055.619635
GGATAGG14150.055.4095346
GATAGTG11350.055.1987957
Produced by FastQC (version 0.11.5)