FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928147
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5550.057.392124
CGTATAG10200.057.3086435
GGATAGG23750.056.594146
GGGATAG21400.056.4298865
CGGGATA9700.056.2933044
GATAGGT22250.054.8977787
GGATAGT21900.054.8218776
TATAGGG26150.053.8038987
CGGATAG9650.053.683185
CTATAGG13000.053.3120846
CGATAGT12250.053.147246
TATAGCG20000.053.023947
ACGATAG8300.052.7151875
GCGATAA10500.052.3376465
CAGATAG12650.052.2968035
GCGATAG12000.052.2126355
GATAGGG20650.052.2023477
GTATAGG22600.052.1945726
CCGATAA7700.051.8224565
GCGTATA9350.051.6620144
Produced by FastQC (version 0.11.5)