FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2807577
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA11200.058.1271174
CAGATAG13750.057.783925
CGGATAG10250.057.7094155
GGATAGT18100.057.2396476
GTATAGG18550.056.228466
GATAGGG17200.055.5513767
GATAGTC10550.055.40197
CGCGATA6050.054.9606744
CGTATAC3950.054.9387055
CGTATAG11000.054.7292635
CGATAGT11850.054.643346
GATAGGT20700.054.6125957
GGATAGG20750.054.315236
GGTATAG16300.053.8976635
GCGATAA9950.053.469285
GATACGG4300.052.9060757
CGATAGG9800.052.8590666
GCGTATA11000.052.8201034
GATAGGA21550.052.7833257
CCGTATA6800.052.5019154
Produced by FastQC (version 0.11.5)