FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593448
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5700.059.5611044
GATAGGA33850.059.0407267
TGATAGG28800.058.6987576
CAGATAG11150.057.7586755
TCCCGTC8850.057.740949
GATAGTC10200.057.3047457
CTATAGG11850.057.30046
GGATAGG15400.057.2735446
CGGATAG9550.057.1735955
GCGATAA8700.057.1272545
GATAGGT15500.056.4524237
CTAGGAC7900.055.823588
GATACGG3850.055.4553387
GGATAGT18700.055.4018636
CCCGTCC10750.055.3496310
CCCGATA3550.055.2114
GATAGGG15350.054.9519277
GTATAGG15400.054.5462346
GGGATAG16400.054.421515
CGGGATA7600.054.3418354
Produced by FastQC (version 0.11.5)