FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466458
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9000.060.671785
GTATAGG15150.057.760646
CGTATAG8550.057.3147745
CAGATAG11350.057.0532655
CGCGATA6100.056.8080644
TATACGG6450.056.4331447
GCGATAA10050.056.422665
CCCGATA4800.056.1505664
GATAGTC8750.055.9990437
TATAGCG9150.055.4635357
TGATAGG19000.055.452046
CGATATG10500.055.3386
CCTATAG6300.055.004645
ACGATAG9100.055.004635
GTGATAG16450.054.685465
CGGGATA8800.054.493234
GATAGGT17900.054.3566137
GATAGGA20450.054.2533537
CGATAGT9500.054.162466
GGATAGT16550.053.932046
Produced by FastQC (version 0.11.5)