FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637304
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4800.060.525514
CCCGTCC6000.058.331210
CTATAGG13250.057.325186
CAGATAG12100.056.9878735
CGGATAG8600.056.9811445
GCGATAG9400.056.227745
GTATAGG19300.056.2219546
GCGATAA9200.055.5477645
GGATAGG18550.055.4759836
TGATAGG23150.055.1878436
GGATAGT19550.055.1449246
GATAGGT19450.055.0622527
TATAGGG21350.054.588177
GATAGTC11500.054.476277
CGGGATA9550.054.245024
CGATAGT11600.054.0127946
GGTATAG15600.053.8503155
CCTATAG6150.053.5000655
GCGCTAC2500.053.2041055
GATAGGA24600.053.0671657
Produced by FastQC (version 0.11.5)