FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833176
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5900.059.9156464
CGGGATA9200.058.9677734
GCGATAA9800.058.928965
TATAGCG8450.058.814887
CGGATAG9750.058.154235
GGATAGT17200.057.5875856
CAGATAG14750.056.9495245
CGATAGG9000.056.389266
CGTATAC4050.056.173215
GATAGTC9300.056.0736587
GCGATAG10800.056.065185
GTATAGG17300.056.040836
CGATAGT10300.055.728526
CGTATAG9750.055.641395
CTGATAG13350.054.7943655
CCTGATA7950.054.591554
CCCGTCC3950.054.04907610
GGATAGC5900.053.9834026
CGATAGC6300.053.889246
AGGATAG11450.053.799485
Produced by FastQC (version 0.11.5)