FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843807
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6100.061.9666184
TCCCGTC16150.061.5473989
CAGATAG14050.060.782345
TGATAGG31500.060.666096
CGTATAC4700.060.3185735
CCCGTCC19450.060.28220410
CTATAGG14850.059.8647426
GATAGGA44150.059.851157
CCGCTAC2950.059.321475
CCTATAG7200.059.304995
GGATAGG19850.058.891136
CGGGATA9000.058.721664
GGTATAG16800.058.124455
GTATAGC8250.058.120666
GATAGGT12650.058.101197
GCGATAG10850.058.0639655
GATAGTC13000.057.8830457
GTATAGG21100.057.558696
CACGATA7150.057.272184
CGGATAG12000.056.874465
Produced by FastQC (version 0.11.5)