FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624889
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4800.061.9777154
CCGATAC2200.060.4531335
GCGATAG10000.059.84865
CTGATAG15400.059.7713285
CGATAGG10150.057.929686
GATAGTC9450.057.7764247
CGGGATA7850.057.5145764
CCGTCCG553.6379788E-1157.273571
CGGATAG7350.057.1415185
GTATAGG13700.056.7146
GCGATAA8700.056.320525
CCTGATA7650.056.2731974
GATAGGA22350.055.904737
CCGATAA8150.055.8269165
TGATAGG19800.055.6805156
CGATAGT11700.055.6397256
CGTATAC2850.055.261865
GGATAGC5150.055.0472566
CAGATAG10750.055.0219655
CGATAAT12350.054.978476
Produced by FastQC (version 0.11.5)