FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481399
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG5550.059.9095845
GGATAGG17300.058.872516
CGGATAG8600.057.383415
CGTATAG8200.056.7679825
CAGATAG10550.056.7295465
GGATAGT17600.056.2781036
GATAGGG15950.055.736377
GCGATAG9750.054.922785
CGGGATA8250.054.726974
GGGATAG17500.054.5997055
CTATAGG11900.054.411476
CCCGTCC5700.054.03479410
GATAGGT16850.054.0056427
GCGATAA8500.053.940885
GTATAGG17150.053.6731766
GGTATAG15050.053.488085
GATAGGA19700.053.4768687
TCCCGTC5500.053.4542549
TATAGGG19200.053.4111637
CTATAGT13250.053.094056
Produced by FastQC (version 0.11.5)