FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766963
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG17500.058.6004686
CGGATAG9000.057.944915
GGATAGT19050.057.3232966
CGCGATA6500.057.0773774
CGTATAG10000.057.0504575
GGATAGG17750.056.1976366
GATAGGA20750.056.1691257
CCTATAG7950.055.472145
TGATAGG19100.055.340766
CCTGATA6400.055.2348184
GATAGTC9600.055.052537
GGGATAG18650.054.6116945
CGATAGT9250.054.486926
GCGATAG10150.054.4831925
GATAGGT17650.054.1364147
CAGATAG12100.054.0913435
CCGATAA8450.053.846585
GCGTATA10350.053.7685474
CTGATAG14400.053.7157065
CGGGATA9800.053.5718574
Produced by FastQC (version 0.11.5)