FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3031531
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGGA40850.060.48837
CGCTCCC1700.059.7095266
GGATAGG21000.059.3369566
GCGATAG12900.058.878595
TGATAGG29650.058.789426
CCGCTAC2750.058.548065
CAGATAG14100.058.087695
CGCGATA8450.057.990754
CCTATAG8050.057.8286675
GTATAGG20300.057.7621466
GATAGTC12200.057.6626977
CGGATAG11750.057.491925
CTATAGG15500.057.358346
GATAGGT18800.056.5945367
GGTATAG15650.056.360345
TCCCGTC11250.056.3108339
CCCGTCC12750.056.2742310
CGGGATA10550.056.068854
GGGATAG18650.055.9274255
ACGATAG8950.055.924285
Produced by FastQC (version 0.11.5)