FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630337
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA7950.060.316964
CGGATAG12300.059.7585685
CGGGATA13500.058.3357434
GGATAGT24050.057.3412446
CAGATAG16800.057.0856975
GTATAGG20800.056.7090766
GGATAGG24150.056.6690066
CGATAGG12250.056.002316
CCCGTCC5200.055.86384610
GCGATAG12450.055.664955
GGGATAG24100.055.4794735
GCGATAA13050.054.9831165
GATAGGG23050.054.965957
GATAGGT25400.054.8410077
CCTGATA9700.054.4867864
TATAGCG11150.054.3034327
GATAGGA28000.053.8735167
CGATATG16600.053.556436
ACGATAG11200.053.439715
AGGATAG16250.053.417595
Produced by FastQC (version 0.11.5)