FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2708265
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC5750.060.25994510
CGCGATA6700.059.5546264
GTATAGG17050.056.6591646
GATAGGT19250.056.5445867
GGATAGG19150.056.111916
CGGATAG9550.056.0755465
GGATAGT19850.056.0727776
GGGATAG18200.055.9637835
GCGATAG10400.055.8676265
CAGATAG13350.055.8449335
CGTATAC3700.055.8130465
GCGATAA10100.055.7943155
GGTATAG16550.055.410085
CGGGATA9350.055.4032944
GATAGGG18400.055.352417
GATAGGA24750.055.0092627
CGATAGT11800.054.2818376
CTGATAG16200.054.014515
AGATAGG19800.053.91636
TCCCGTC6000.053.6658489
Produced by FastQC (version 0.11.5)