FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298366
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA7500.059.2671974
CGGATAG12250.059.1433835
GGATAGT20700.058.672026
CTGATAG16850.057.122175
CGGGATA10100.056.4856534
CCTATAG8950.055.922285
GTATAGG20500.055.829766
CGATAGG10800.055.7412346
GCGATAA13450.055.688235
GGATAGG20100.055.5477526
TATACGG9000.055.221047
CAGATAG16150.055.0469325
GGTATAG19850.054.836765
ACGATAG9600.054.687995
CGTATAC5150.054.3694155
GATAGGG20900.054.089757
GATAGTC10950.054.0171057
GCGATAG12650.053.6763655
CGATATG15350.053.5835386
GGGATAG19300.053.4978875
Produced by FastQC (version 0.11.5)