FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519216
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTC9350.061.014979
GCGATAA10400.059.570995
CCGCTAC2000.059.5036775
TGATAGG22200.058.6534356
CGCGATA6850.058.2517784
CGATAAC5350.058.2290576
GATAGGA26550.057.6072777
GGATAGG15250.057.3817336
CCCGATA5250.057.336884
GATAGGG16200.057.036037
GGTATAG14000.057.0035255
CCCGTCC11550.056.96982610
TATAGGG21900.056.7341657
CGCTATA8100.056.176314
CTATAGG13300.055.7940336
CGGGATA8350.055.7519534
ACGATAG8850.055.370655
GATAGTC11650.054.9775737
GTATAGG17900.054.948626
CGGATAG9650.054.7702265
Produced by FastQC (version 0.11.5)