FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503567
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA8450.059.646064
CGGATAG12000.058.0427325
GTATAGG22050.056.98686
CGGGATA12450.056.788194
GGATAGT24250.056.7243086
GGATAGG22250.056.4745566
GATAGGG20700.056.4736637
CGATAGG12200.055.6583486
CAGATAG15550.055.370795
CGTATAG11850.055.233085
GGTATAG21500.055.187065
GCGATAA13400.054.5905535
TATAGCG11800.054.2798847
GATAGGT23850.054.151167
CTATAGG17150.054.0841676
GATAGTC12500.054.0402227
GGGATAG21700.054.033255
GCGATAG12800.053.594735
CGATATG14700.053.335846
TATAGGC17550.053.248087
Produced by FastQC (version 0.11.5)