FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3780180
Sequences flagged as poor quality0
Sequence length76
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG12950.057.8427246
TATAGGG24150.057.6814547
GGATAGG21600.057.3659556
GGATAGT26500.057.1935126
GATAGGG18450.057.100567
CGCGATA8600.056.9808044
GGTATAG20750.056.8474275
GATAGGT26550.056.6857877
GGGATAG21000.056.1706665
GTATAGG21250.056.1694456
CCGATAG6800.056.1069375
CGTATAG12200.055.946615
TATAGGT32600.055.9358677
GCCGATA7450.055.9100234
GCGATAA12950.055.6796385
CGGGATA10750.055.352784
CAGATAG12150.055.3125845
GCGTATA12650.055.0632134
ATAGCGC3000.054.833618
CCGCTAC2750.054.7311675
Produced by FastQC (version 0.11.5)