FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22406106
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16449287.341427376983756No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCTACACTCTTTCCCTACAC478980.21377208516285695Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258660.11544174610260258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCTA119150.064.5290628
CCAGTCT124600.063.10935227
GATATAG28700.061.94289462
CGATATA31400.061.5212161
TCTACAC92650.061.07842331
TACACTC81100.059.857933
TATTCCG20700.059.68049656
CTACACT90050.058.76705632
TCCGATA45600.057.944159
ATATAGA33100.055.4044863
TTCCCTA111850.053.63262641
TAGATAG33500.053.59961766
TCCCTAC98250.053.14879242
AGATCGG298350.052.880991
CACGCTA13650.052.8136551
CGCTATT23450.052.2324853
CTAGATA20400.051.98711865
CCCTACA106100.051.9540543
TTTCCCT114300.051.65546840
ATCTAGA63700.051.53532863