FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_RA1_Strep_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_RA1_Strep_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6973639
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140740.20181715744104334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2352.774268E-614.89446344
ACCTTCT13850.013.3957993
TGGGGGG10850.012.5824411
TCTCGTA2601.17747295E-412.11598641
GTATGCC2952.8943656E-511.8650845
GCTTGAA3953.5711128E-711.51906359
ATCTCGT3358.374111E-611.49261340
TCTACCT8600.010.5826792
CTAAGTA2700.00218413610.3718511
AGTCACC3451.4239049E-410.14441228
ATGTCCG2900.0039484669.6565521
CCGATGT3400.00140195569.26436833
GATCGGC20100.08.88040113
ATCGGCG20100.08.88040114
TAGCATA3200.0088816068.74999538
CCGTCTT3700.0030217528.51350950
GGCGTTC21450.08.48478217
CCGGTCC20900.08.3727823
CTTGAAA5502.4393337E-58.27254460
TCCGGTC21650.08.24438522
Produced by FastQC (version 0.11.5)