FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_RA1_Strep_ChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_RA1_Strep_ChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293819
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG579410.5628717582852389No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC437400.4249151845393823TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG179620.17449306229301292TruSeq Adapter, Index 1 (100% over 49bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111770.10857972148140549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC79550.042.1516844
TCTCGTA77050.041.83894741
GTATGCC82750.040.5641445
CTCGTAT81400.040.20547542
GTCACAT84250.039.83786829
TCGTATG86000.039.31590343
ATCTCGT82750.038.87050640
GCTTGAA88050.038.3589259
CACGATC84700.037.81013536
CTTGAAA90250.037.34593260
CCGTCTT91650.036.7379850
CGTCTGA96650.036.57530616
TCACGAT91400.036.22383535
ATCACGA92950.036.07161734
ACACGTC98700.035.9581813
CACACGT100800.035.2075112
TGCTTGA96500.034.96410458
TCACATC97350.034.62101430
GCACACG104250.034.0761111
CTCCAGT103350.033.82986524
Produced by FastQC (version 0.11.5)