FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_RA1_3unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_RA1_3unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6088187
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198080.32535137307707535No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC118410.19449139784963898TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28250.034.19727743
TATGCCG28250.033.57748846
CGTATGC29150.032.7812144
CTCGTAT29700.031.5851442
ACGTCTG35750.030.0557515
CGTCTGA35500.029.67610716
AATCTCG31700.028.92749639
ACTCAAT33450.028.35440635
GTATGCC35000.027.50208345
CACGTCT40100.026.70805514
AGTCACC37950.025.54555928
ATCGGAA42350.025.2903392
CACACGT42600.025.221612
AAAAGGG36600.025.14928470
CACTCAA38650.024.99234434
GATCGGA42800.024.9430661
TCGGAAG43550.024.7556423
CAGTCAC40450.024.22629227
CCACTCA39500.024.10012233
GTCTGAA44750.024.01121517
Produced by FastQC (version 0.11.5)