FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_RA1_3sorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_RA1_3sorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7744180
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATG81975610.58544610275071TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC6148547.939562355213852TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATATCGTATG152430.1968316852139284TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATATCGTATGC132160.17065719030291135TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGAGCTCGTATG99190.12808328318814904TruSeq Adapter, Index 7 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95040.12272442014519291No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGAGCTCGTATGC77690.10032049874873776TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG696850.066.4046170
AGATCGG1027350.065.0429841
ACACTCT60750.044.47510534
CGCTAAT15000.044.33222653
AGTCTAC32650.043.73381429
ACTCTAT39600.042.24778436
GTCTACA34250.041.6907730
AACACTC35600.041.19172733
CTCTATC42200.039.97659337
CGTCTGA1711250.039.18852217
ACGTCTG1711600.039.1723316
CACGTCT1714950.039.11697415
GTCTGAA1716600.039.05951318
CACACGT1721800.039.01648713
CTCGTAT1570250.038.99074643
CGTATGC1617950.038.98397445
TATGCCG1617700.038.974647
ACACGTC1723100.038.9647114
CCCTAAA21200.038.9643143
TCGTATG1617850.038.94960444
Produced by FastQC (version 0.11.5)