FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_RA1_2unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_RA1_2unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7130523
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA201380.2824196766492444TruSeq Adapter, Index 22 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93600.13126666865810543No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT76560.1073694033382965TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA34450.042.77264437
TCGTATG36950.040.06869546
TATGCCG37650.039.50882749
CGTAATC36950.039.02562739
CGTATGC38500.038.2737247
CTCGTAT39850.036.7141445
ACCGTAC40950.036.23898733
ACGTAAT41200.035.6800438
CGTCTGA43650.035.03977217
GTATGCC43300.034.19161248
ACGTCTG45150.033.9531816
CACCGTA45250.032.872432
CACGTCT47750.032.03179615
GTAATCT45750.031.82484640
AATCTCG46300.031.44812242
AGTCACC47550.031.20787629
TAATCTC47600.030.66256141
CAGTCAC49750.030.10922228
CCGTACG49700.029.85888334
AGATCGG51950.029.3106631
Produced by FastQC (version 0.11.5)