FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_RA1_2sorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_RA1_2sorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7282231
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATG97530113.392887426943748TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC75269010.335980827853442TruSeq Adapter, Index 7 (97% over 35bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75350.10347103792779987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG817850.066.6554770
AGATCGG1149850.065.039241
ACACTAT28900.046.02017234
AAAGGGG34550.044.57159470
CGCTAAT8850.040.73422253
TACTCTA21000.040.1719444
ACTCTAG21200.039.958355
CGTCTGA1930400.038.7888717
AGTCTAC22650.038.78457329
ACGTCTG1932100.038.73316
CACGTCT1935350.038.6842415
GTCTGAA1936000.038.6727918
TATCTCG1842200.038.5767540
CGATATC1842300.038.56949237
ACGATAT1861850.038.5461136
CACACGT1943350.038.53759413
AACTCCA1937450.038.5135223
CGTATGC1865200.038.50555445
CTCGTAT1849900.038.5023843
TCGTATG1866800.038.5006744
Produced by FastQC (version 0.11.5)