FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_RA1_1unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_RA1_1unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8674052
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18250.015.34254348
AGTCACG17600.014.91407528
TCGTATG19150.014.80519145
GGTCCTA18400.014.45742746
CTCGTAT19800.013.96560244
TTAGGCG30800.013.862987533
CGTATGC20650.013.72967346
GTCCTAT21150.012.57754847
CTATTAG21550.012.50593550
GTACCGA33200.012.22904816
CCCGTAT10500.012.0008911
ACGTCTG25700.011.98463315
CGGGAAT41750.011.4858821
TACCGAG35800.011.43867117
CCCGGTT15000.011.4341811
CTCGGTT19350.011.2153021
GTATGCC26000.011.17371447
TTAGTAA25500.011.11744753
GGTACCG38250.011.07207915
CGTCTGA26000.010.76934716
Produced by FastQC (version 0.11.5)