FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_Strep_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_Strep_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7040432
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130730.18568462844325462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2704.40632E-815.55610544
TGGGGGG12300.014.7977951
ATCTCGT2851.3722747E-613.50905740
TCTCGTA3054.070042E-511.47581541
GTATGCC3409.8699875E-611.3239345
ACCTTCT13950.09.7858483
TCTACCT9101.8189894E-129.2313572
GCTTGAA3955.539281E-48.86056959
AGTCACC4351.4815458E-48.85019628
CTTGAAA5502.1910513E-47.63625460
GATTCTA20700.07.439400764
CGATTCT20900.07.3682163
CGTCTGA10503.3905962E-97.333071770
GATCGGC20300.07.24158413
GGCGTTC20050.06.98264117
TGATCGA23200.06.939506559
TGCTCCC22250.06.921592750
CTGTGAT22850.06.89272956
CCCTGTG23050.06.83287454
TCCCTGT22600.06.81401353
Produced by FastQC (version 0.11.5)