FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_Strep_ChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_Strep_ChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4826556
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83070.17211029976654163No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3400.017.5003414
CACACGT3655.7480065E-1014.38384113
CGTATGC2253.0347124E-514.001142545
TCTGAAC4303.274181E-1113.83733519
GCACACG3951.877197E-913.29070912
GACGCAA1850.001549610413.24308838
CGTCTGA4406.87578E-1012.7275217
CACGGTA3003.4400775E-511.66616732
CTGAACT5105.657057E-1011.66604720
TCTCGTA2650.001870585410.5667942
GTAGCAT3004.4442163E-410.49987736
AGCACAC5754.112735E-910.34749111
AGAGCAC6101.0833901E-89.7541879
ATCTCGT2950.00454945549.49210341
GTATGCC3400.0014002839.26546145
ACCTTCT9202.0263542E-97.99003033
TCGGAAG7151.0579626E-57.34348444
TCTACCT6954.394416E-46.54716062
TGGGGGG9953.807505E-66.33243751
TGCTTGA6750.00225995346.222149458
Produced by FastQC (version 0.11.5)