FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_3unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_3unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9669809
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG26550.014.36835633
AGTCACG16250.012.49158329
TCGTATG16500.012.30378144
CGGGAAT34150.012.1973151
GTACCGA31400.012.1499216
CCCGTTA4953.43789E-1012.0213081
GTTACGA10850.011.6139124
TATGCCG17850.011.37306547
CTCGTAT18400.011.03328143
CTTAGGC36250.010.81322232
TACCGAG35950.010.80688717
GGTACCG37050.010.67508315
GGTCCTA15400.010.45507446
GAATTGG43200.010.3712714
TAGGGGC28800.010.32931623
ATTCTTA38450.010.2854929
GGAATTG45100.010.0121623
TCTTAGG41000.09.816504531
ACGTCTG23350.09.74323216
CGGTACC39900.09.737082514
Produced by FastQC (version 0.11.5)