FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_3sorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_3sorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8159375
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG818691.003373420145538TruSeq Adapter, Index 10 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC169350.20755266181539642TruSeq Adapter, Index 10 (100% over 50bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT95970.11761930294906166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC119550.051.7647445
CTCGTAT118500.051.63297343
TCGTATG121400.050.83205844
TATGCCG122350.050.46566447
GTATGCC122750.050.41527646
AGTCACT127150.049.7920229
CGTCTGA152250.049.68075617
TATCTCG123600.049.58501440
ACGTCTG153300.049.31764616
TAGCTTA127900.049.19994735
AGATCGG154900.048.8531231
CACGTCT157400.048.16731615
GTCTGAA159700.047.297418
CACACGT161400.047.0600413
TCACTAG134800.046.9931131
CACTAGC135850.046.52684832
TCGGAAG165800.045.6628234
CAGTCAC140050.045.4305828
TTATCTC135550.045.3424639
ACACGTC169050.045.03423714
Produced by FastQC (version 0.11.5)