FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_3Nsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_3Nsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7229246
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT89809712.423107472065551TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA84765911.725413687679186TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTA128140.1772522334971033TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATATCGTAT110320.1526023599141598TruSeq Adapter, Index 21 (97% over 40bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT89860.12430065320781723No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAACAATCTTAACCTACAA85640.11846325329086878TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTAT84530.11692782345489418TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTA80500.11135324486122065TruSeq Adapter, Index 21 (97% over 40bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74020.10238965446742303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG1033500.065.1373870
AGATCGG1080500.064.279921
CCTACAA46950.049.50135844
AACCTAC24750.047.51696442
TTACCTA21400.043.5063341
AATACCC21200.042.4296949
GATATAA32650.042.34142762
CAGATAT30650.042.25049660
AGATATA30750.042.22633461
ACTCTAG29000.042.126965
TACCCTC24250.041.9989951
CGATATA15700.041.68660761
ACAATCT54400.041.23924634
ACCTACA47450.041.16068343
TACTCTA30000.040.7221644
GTCAACA42150.040.18771730
AGTCTAC37250.040.11872529
ATACCCT24000.039.66708450
GTACTCT31300.039.031913
CCCTCTT84900.038.9984253
Produced by FastQC (version 0.11.5)