FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_2unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_2unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6197608
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG13950.018.81797643
CTCGTAT13300.018.68527842
TATGCCG14350.018.29328746
AGTCACG14350.017.8040328
CGTATGC17200.015.26250144
ACGTCTG18900.014.44527115
TATCTCG18000.014.19468439
CGTCTGA18300.014.153357516
TTAGGCG18600.013.3594733
GTATGCC19900.013.19149745
CGCCAAT19200.013.12426933
CACGTCT21000.012.83406714
GTACCGA20250.012.61759916
AAAAGGG20750.012.31266570
CGGGAAT25500.012.0797071
ACGCCAA21500.011.88315532
CCCGGTT10150.011.3805121
GGTCCTA12650.011.34424746
CTATTAG13000.011.30797450
CCCCCCT10950.011.18839551
Produced by FastQC (version 0.11.5)