FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_2sorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_2sorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6032515
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG348320.5774042832881476TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC131300.21765383094778878TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG64650.047.5922161
CTCGTAT60600.043.83961543
TCGTATG61700.043.62569444
TATGCCG61550.043.33321447
CGTCTGA71500.043.2252917
CGTATGC62700.042.70660845
ACGTCTG73200.042.60394716
CACGTCT73650.042.05814415
GTATGCC64650.041.68849646
CACACGT79200.039.24379313
AGTCACC70550.039.23881529
GTCTGAA78900.039.21556518
ATCGGAA78700.039.1860243
CAGTCAC70800.038.95196528
TCGGAAG80100.038.500494
GATCGGA80650.038.2787932
AAAAGGG28500.036.22573570
ACACGTC87100.035.7647414
AGCACAC88750.035.05747611
ACCAGAT78400.034.9528133
Produced by FastQC (version 0.11.5)