FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_2Nsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_2Nsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5847492
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG413900.7078248247282767TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC137720.23551977497361262TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67350.046.40859647
CTCGTAT66400.046.1237543
CGTATGC68050.046.0859145
AGATCGG78550.045.9457471
TCGTATG68100.045.7950944
GTATGCC71150.044.1267746
CGTCTGA81050.044.04705417
GTCACAC75600.043.2849930
ACGTCTG82500.043.23047316
CACGTCT84150.042.5499115
AGTCACA76850.042.53539729
TCACACT76800.042.47194731
GTCTGAA85950.041.61738218
CAGTCAC79200.041.22910728
CACACGT87250.040.91776713
TCGGAAG88800.040.523724
ATCGGAA89100.040.465853
GATCGGA91900.039.383622
AATCTCG78700.038.95855340
CACACTT83350.038.9243732
Produced by FastQC (version 0.11.5)