FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_1unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_1unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7882635
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143110.18155096614266678No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA101570.1288528518699648No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG87340.11080051277269592TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG23200.026.85208333
TATGCCG24550.025.09339547
TCACTTA25650.024.69628531
TCGTATG26000.024.2326144
CGTATGC26550.023.73061845
CACTTAG27100.023.24604832
CTCGTAT28000.022.1265443
TTAGGCA29200.021.45438835
GTATGCC29850.020.98973546
GTCGCTA41000.019.88897727
AGTCACT31800.019.8100629
CGCTATG40800.019.72913729
CTAGGGC18600.019.38110251
CCTAGGG18150.019.28411150
CGTCTGA36150.018.686517
GTCACTT34900.018.35126930
GCTATGA45050.018.02327230
ACGTCTG38250.017.93520716
GCAACGT45550.017.74859622
GCGCCTA20650.017.45884347
Produced by FastQC (version 0.11.5)