HFHGMBGXC_n01_NLS_1sorted.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HFHGMBGXC_n01_NLS_1sorted.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5941778
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG	280630	4.722997055763443	TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC	108603	1.827786228297321	TruSeq Adapter, Index 4 (100% over 50bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT	9708	0.16338543782685924	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATG	8919	0.15010658425811263	TruSeq Adapter, Index 4 (97% over 49bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC	8415	0.14162427475412243	TruSeq Adapter, Index 4 (100% over 50bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA	8231	0.13852755858599902	No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG	7043	0.1185335433265935	No Hit

Adapter graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AAAAGGG	14900	58.41809	70
AGATCGG	40730	56.795265	1
TACTCTA	3375	47.085526	4
TATGCCG	45065	46.879433	47
ACTCTAG	3360	46.87903	5
TCGTATG	45235	46.86653	44
CGTATGC	45295	46.819504	45
GTATGCC	45330	46.659424	46
CTCGTAT	43765	46.592896	43
CTTGAAA	46385	45.785664	61
ACTGACC	46310	45.69278	33
TTGAAAA	46665	45.630173	62
CCGTCTT	46325	45.59526	51
AGTCACT	46710	45.54126	29
CCAATCT	44490	45.439507	38
ATGCCGT	46610	45.3773	48
GCTTGAA	46695	45.376766	60
AAAAAGG	45065	45.270214	70
CACTGAC	46920	45.22554	32
ACCAATC	44720	45.197598	37