FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_NLS_1Nsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_NLS_1Nsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6015292
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1443152.3991354035681063TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1098721.8265447462899556TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG132050.061.30409670
AGATCGG225050.055.49711
CTCGTAT277300.038.76211543
CGTCTGA322900.038.65303417
ACGTCTG324200.038.5412316
TCGTATG286300.038.5090844
TATGCCG287100.038.4505447
CGTATGC288750.038.3277945
CACGTCT327450.038.14864315
GTCTGAA327300.038.12303518
GTATGCC290700.038.1188546
AGTCACA300100.037.6341129
GTCACAC299450.037.63398730
CAGTCAC301350.037.5012328
CACACGT334950.037.3783513
CACAGTG301450.037.1982334
AGTGATC290000.037.08812337
CAGTGAT298100.037.03108236
ACACGTC338350.037.02312514
ACACAGT305050.036.8051333
Produced by FastQC (version 0.11.5)