FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_Strep_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_Strep_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7094827
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111260.1568184819728515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2405.0386006E-1018.9595644
TCTACCT8600.014.6521112
CCGTCTT2951.3043064E-714.23770750
GACGATT1500.00593834714.00031517
GTATGCC3354.1678504E-813.5829745
AGTCACC4455.820766E-1113.37023828
TCTCGTA3152.901852E-713.33400841
GCTTGAA4406.87578E-1012.72737959
TTGCGAC2000.002784711512.24949834
CTAATCT3501.0411277E-612.00035437
TGGGGGG11550.010.91002751
CCAACTA4056.798521E-59.50578333
TCTGAAC7655.4023985E-109.15053318
CGTCTGA8101.6552804E-109.07427816
ACACGTC8302.6921043E-108.85568313
CAACTAA4905.3541304E-47.856820634
CACACGT9655.144102E-97.61626812
GCACACG9553.4100594E-87.329490711
CTTGAAA6854.7666763E-57.15329460
GATCGGC17300.07.08108415
Produced by FastQC (version 0.11.5)