FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_Strep_ChIP.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_Strep_ChIP.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10658812
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG622620.584136393436717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC129140.12115796769846396TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28700.033.05106744
TCTCGTA30000.030.33514641
GTCACAT33100.030.02862529
GTATGCC33100.028.76331745
GCTTGAA33950.028.2480559
ACACGTC38450.027.12648813
CGTCTGA38300.027.04970416
CACACGT38600.027.0196812
TCTGAAC38700.026.6796818
CTTGAAA36250.026.45526360
GCACACG40700.025.53955311
CTCGTAT37450.025.51578342
CCGTCTT36700.025.46366350
TCGTATG38200.025.1979643
AGCACAC43500.023.97619610
AGAGCAC44450.023.4644288
CTCCAGT47000.021.89256324
ACTCCAG48400.021.54844923
GTCTGAA48250.021.54411717
CACATGA46750.021.11108631
Produced by FastQC (version 0.11.5)