FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_3unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_3unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9249443
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCG26300.014.37199533
TATGCCG18650.013.5123549
CGGGAAT35050.012.8827751
GTACCGA30500.012.73797416
CGTCTGA23200.012.67268117
CGTATGC19550.012.53277647
TCGTATG18550.012.26493946
ACGTCTG24500.012.00025416
CTCGTAT19300.011.96974745
TAGGGGC22250.011.48276723
TACCGAG35150.011.3515917
CTTAGGC33700.011.21612632
TCGAATT36900.010.71786125
GGTCCTA14300.010.52515246
CACGTCT28350.010.49404815
GTATGCC24350.010.49303448
CCCCGGT20800.010.4336451
CCCGGAT23500.010.4264841
CGGTACC40000.010.23771514
GGTACCG37950.010.14514215
Produced by FastQC (version 0.11.5)