FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_3sorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_3sorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5743934
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG388610.6765572167089663TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC262410.45684717129409913TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG44800.045.3110170
AGATCGG82800.043.5844151
TATGCCG79850.037.34671447
TCGTATG80250.036.94248644
CGTATGC81450.036.6130845
CTCGTAT80300.036.48359743
CGTCTGA100200.035.97873717
AGTCACG82550.035.91007229
GTATGCC82750.035.868746
ACGTCTG101750.035.4306616
CACGTCT104050.034.64747215
CACACGT106950.033.77373513
GTCTGAA108500.033.3877418
GTCACGT89700.032.93062630
ACGTAGA89600.032.8895433
CAGTCAC91700.032.8230628
ATCGGAA111550.032.3841063
TCGGAAG113050.032.015234
CGTAGAG92050.032.01415334
GATCGGA113850.031.758692
Produced by FastQC (version 0.11.5)