FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_2unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_2unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6396443
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG18450.021.62725845
CGTATCT18300.021.42082239
TATGCCG19450.021.23432448
TTCCGTA19300.020.67346836
CTCGTAT19350.020.4404544
CGTATGC20650.019.4923446
TATCTCG20450.019.34035941
TCCGTAT20300.019.31024637
AGTTCCG20750.019.22806434
CCGTATC20950.018.54405838
GTTCCGT21500.018.39453535
CGTCTGA25000.017.50059316
GTATGCC23200.017.34972847
ACGTCTG25500.017.15798215
CACGTCT28950.015.23403514
GTATCTC26100.015.15341640
AGTCACA27600.015.08991128
TTAGGCG17250.013.9993833
GTCTGAA34200.013.40688717
CAGTCAC32550.013.22532527
Produced by FastQC (version 0.11.5)