FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_2sorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_2sorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8600982
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA6710437.80193470931575TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT3704294.306822174491239TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCAAAACTCATAACCTACAA109820.1276830947908041TruSeq Adapter, Index 1 (97% over 34bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT107070.1244857854603114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG879300.062.7882351
AAAAAAG493900.059.91391470
AACCTAC33850.054.28804842
CCTACAA52250.051.7171544
ACCTACA47950.045.69717443
TCATAAC38200.045.07933838
AATACCC23500.044.5321949
CGTCTGA1247500.044.32893817
ACGTCTG1248800.044.26571316
CACGTCT1251500.044.1849715
GTCTGAA1253150.044.1793418
TATGCCG1120350.044.152149
TCGTATG1115850.044.10688446
CTCGTAT1103900.044.0904845
CGTATGC1122700.044.07134647
ACTCTAG33450.044.054175
GTATGCC1128350.044.00346848
CTCCAGT1250750.043.90363725
TCCAGTC1248800.043.88250426
AGTCTAA25650.043.7991529
Produced by FastQC (version 0.11.5)