FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_1unsorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_1unsorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7536403
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC350850.465540391085774TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG234130.31066544610207286TruSeq Adapter, Index 12 (100% over 49bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174410.23142339919985702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG54050.045.3914770
TCGTATG70800.035.00079343
CTCGTAT69350.034.9755642
TATGCCG72600.034.5184646
CGTATGC72600.034.27763444
GTATGCC75050.033.34519245
CGTCTGA82450.031.41223116
AATCTCG77700.031.30597739
TGTAATC78800.031.09104336
ACGTCTG85100.030.88666215
TAATCTC79550.030.53393438
CACGTCT86900.030.00523814
AGTCACC84800.029.5507928
GTAATCT83000.029.51776337
CAGTCAC87150.028.79411127
GTCTGAA91550.028.25164817
AGATCGG69250.027.3466661
CTTGTAA92700.027.07037534
CACACGT97000.026.8086412
TCTCGTA91550.026.76193841
Produced by FastQC (version 0.11.5)