FastQCFastQC Report
Fri 6 Sep 2019
HFHGMBGXC_n01_KN1_1sorted.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGMBGXC_n01_KN1_1sorted.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6208869
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT4587367.388398756681773TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA4515057.271936322058011TruSeq Adapter, Index 13 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATATCGTA65850.1060579632135901TruSeq Adapter, Index 13 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAG560900.061.78003370
AGATCGG594450.061.3371851
AGTCTAA16550.046.73533229
CCTACAA34200.044.21271544
AACCTAC16900.042.4573842
GTCTAAG10100.041.9293930
TACTCTA22100.041.6574
TATAAAA35550.040.2656564
ACTCTAG22800.040.224535
ACCTACA26300.040.1917843
CTACAAT37400.040.14904445
GCTATAA11850.039.28116662
TACAATG27350.038.9047246
CAGTCTA28200.038.84599728
AGATATA26850.038.32283461
TCTAGTT23650.038.1837047
ACAATGC28650.037.38344647
GTTACCT24500.037.0009414
CCCTCTT57100.036.71514553
TTACCTC24850.036.47979715
Produced by FastQC (version 0.11.5)