FastQCFastQC Report
Tue 9 Jul 2024
HFHGFDRX5_n02_YTW274_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW274_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534447
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA236915452.24791468507626No Hit
CGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAA1555193.4297236245125373No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC1451323.200654897940146No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT1344562.96521273707687No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT1057202.3314860665479165No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG859621.8957548737475596No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA794141.7513491722364378No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG732321.615015017266714No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA488971.0783453858871876No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG425670.9387473268515433No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT388510.8567968707099234No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG267310.5895095917980737No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT224020.4940403978699056No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA219630.48435895270139884No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC215680.4756478573903279No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA213070.4698919184632657No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG212000.4675322040372288No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT166570.3673435812569868No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT163280.3600880107320694No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG162230.3577724031177341No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT153700.3389608479269909No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT141460.31196747916559614No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA139740.3081742933592564No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG136960.30204344653273046No Hit
CATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCAT132220.29159013215944524No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA129880.2864296351903551No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC122440.2700219012373504No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT120310.2653245257911273No Hit
CTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGC119190.2628545443358363No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA109670.24185970196586265No Hit
ATTAACCCTAGAAGGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA100300.22119566068365118No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA93270.20569211637052986No Hit
ATTAACCCTAGAAAGATAGTCCGCGTAAAATTGACGCATGCATTCTTGAA92240.20342061556789617No Hit
AATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAG89970.19841449243976167No Hit
AAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACG86880.19159999003186054No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG82250.18138926312293427No Hit
CCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC79150.17455270730918235No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAAAATGACGCATGCATTCTTGAA78430.17296486208792386No Hit
CAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGAT71340.15732899733969766No Hit
ATTAACCCTAGAAAGGTAGTCTGCGTAAAATTGACGCATGCATTCTTGAA70080.15455026820249526No Hit
ATTAACCCTAGGAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA69960.1542856273322855No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC69270.15276394232857943No Hit
ATTAACCCTAGAAAGATAGCCTGCGTAAAATTGACGCATGCATTCTTGAA65450.144339541293569No Hit
CAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTA63470.13997296693510808No Hit
ATTAGCCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA62720.13831896149629713No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA61650.13595924707026016No Hit
ATTAACCCTAGAGAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59980.13227632829317446No Hit
ACTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59270.13071053647776676No Hit
ATTAACCCTAGAAAGACAGTCTGCGTAAAATTGACGCATGCATTCTTGAA59260.13068848307191594No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAGAATTGACGCATGCATTCTTGAA58560.12914474466235906No Hit
ATTAACCCCAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA58460.12892421060385093No Hit
ATTAACCCTAGAAAGATGGTCTGCGTAAAATTGACGCATGCATTCTTGAA57480.12676297683047127No Hit
ATTAACCCTGGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA57080.12588084059643878No Hit
AATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAA51810.11425869571306049No Hit
AAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAA51640.11388378781359666No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAGATTGACGCATGCATTCTTGAA50780.11198719491042677No Hit
ATTAACCCTAGAAAGATAGTCTGTGTAAAATTGACGCATGCATTCTTGAA50550.1114799665758581No Hit
ATTGACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA48320.10656205707112686No Hit
ATTAACCCTAGAAAGATAGTCTGCGTAAATTGACGCATGCATTCTTGAAA48190.1062753627950663No Hit
AAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATA47820.10545938677858624No Hit
CACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTT45480.10029888980949606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)