FastQCFastQC Report
Tue 9 Jul 2024
HFHGFDRX5_n02_YTW272_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW272_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19618481
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA202165110.30482941059504No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA9762304.976073325962392No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA7622953.885596443475924No Hit
TGAAACTCAAACTGGGAGGAAGATAAGAAAAGCTTTAACCCTAGAAAGAT6441603.28343463492408No Hit
GCTGTGAATGCTGCGGGCATTCTCCTCCGCAGAGCTCTTTCTTAACCCTA3181551.6217106716875787No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT2719981.3864376146145057No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA2297031.170850077536584No Hit
TAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCT2249651.1466993800386482No Hit
GCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGA2191191.1169009466125333No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG2070321.0552906720963768No Hit
TAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAG2016591.0279032306323816No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA1840760.9382785547973872No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT1734900.8843192294041522No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT1683770.858257068934134No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT1538920.7844236258658355No Hit
TCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATC1448820.7384975421899381No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG1396300.7117268661116016No Hit
TTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAA1351060.6886669768164008No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC1165350.5940062332042935No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG1139460.5808094928450372No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT1056620.5385840014830914No Hit
ACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATAT898340.45790497235744193No Hit
TGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGG865020.44092098669616675No Hit
TAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT815380.4156183141803894No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA756940.38583007522345897No Hit
AAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACG734890.37459067294761506No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC717570.36576226263389094No Hit
ATTGAAGATTGTAAATCTAAATCCAAATTATGAATGATTTTCTTGGCCTC621560.31682371331399206No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG593290.30241383112178766No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC592520.30202134405818676No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG566580.28879911752596954No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA504830.2573236939190144No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA495340.25248641829099816No Hit
TTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA459780.2343606520810658No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC441580.22508368512322638No Hit
TAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGA307330.15665331072268032No Hit
CTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA247620.126217722972538No Hit
TTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCA207490.10576252055396133No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)