FastQCFastQC Report
Tue 9 Jul 2024
HFHGFDRX5_n02_YTW268_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW268_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4915156
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA165071033.58408156323014No Hit
GCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGA1605253.265918721603139No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT1249832.542808407301823No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT1025812.087034470523418No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC903321.8378256966818551No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT828571.6857450709601078No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA541321.1013282182701831No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG434160.88330868847296No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG426130.8669714654021153No Hit
TGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGTCG408540.8311841984262553No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT357700.7277490277012572No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG313570.637965509131348No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC299750.6098483954527588No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT294210.5985771357002708No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG273320.5560759414350226No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG212590.43251933407606996No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA201160.40926473137373465No Hit
TAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT200230.40737262459217977No Hit
ACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATT194520.3957554958581172No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT192600.39184921088974595No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA178960.3640983114269415No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG171730.3493887070929183No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA162370.3303455678721082No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG155910.3172025465722756No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT154010.31333695207232487No Hit
CTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA150700.3066026795487264No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG150510.30621612009873134No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA136840.2784041849332961No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT131130.26678705619923354No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT104720.21305529264991793No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA104170.21193630476835323No Hit
TATTGACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTC103310.21018661462627025No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA101320.20613791301842707No Hit
GCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCT95150.1935849035106922No Hit
TGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGCATGAG87080.1771662995030066No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC83960.17081858642940326No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC80660.16410465914001507No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA78590.15989319565848978No Hit
GAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAATGCCT77060.1567803748243189No Hit
GACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCA73460.1494560905086227No Hit
CTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGA73370.1492729834007303No Hit
CCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGC72310.14711638857444198No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG71860.14620085303497998No Hit
GCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAG69620.14164352057188012No Hit
TTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAA68830.140036247069269No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA65010.13226436760094695No Hit
CTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGC63890.12998570136939702No Hit
GCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTG63350.1288870587220426No Hit
GATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGA62620.12740185662469308No Hit
ATTAACCCTAGAAGGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA61750.12563182124839983No Hit
GCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGT59330.12070827456951519No Hit
GGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAAC58280.11857202497743714No Hit
TAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCT56000.11393331157749623No Hit
GTAGGTCAGGTTGCTTTCTCAGGTATAGCATGAGGTCGCTCTTATTGACC55170.11224465713804405No Hit
TGACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCC55100.11210224049857218No Hit
ATTAACCCTAGAAAGATAGTCCGCGTAAAATTGACGCATGCATTCTTGAA55090.11208189526436191No Hit
CATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCAT54620.11112566925647935No Hit
AAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACG54000.10986426473544278No Hit
TAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGT53970.10980322903281198No Hit
ATTAACCCTAGGAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA52240.10628350351443575No Hit
CGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATT52140.10608005117233307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)