FastQCFastQC Report
Tue 9 Jul 2024
HFHGFDRX5_n02_YTW257_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGFDRX5_n02_YTW257_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14103524
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA292218720.719552077906204No Hit
AAACTTCCTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCAT169734512.034899929974948No Hit
AGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAG148884010.5565105572196No Hit
CCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATT3580002.538372679055249No Hit
GCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG2745831.9469105735559427No Hit
GCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGA2679571.899929407713987No Hit
GTGCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT2445081.7336659972358681No Hit
AGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTT1625601.1526197282324617No Hit
AAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGC1419821.0067129321721295No Hit
GTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGAT1414731.0031039050949253No Hit
AGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGA1240150.8793192396453539No Hit
CTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA1016000.7203873301452884No Hit
CTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCG809640.5740692893492435No Hit
GCATGAGGTCGCTCTTATTGACCACACCTCTACCGGCATGCCGAGCAAAT669510.47471114311572055No Hit
GTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAG663650.47055615319972516No Hit
ATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCGT633940.4494904961341577No Hit
GAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTA589800.4181933536611134No Hit
AGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGA489200.3468636632943653No Hit
AGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTG488440.34632479088205187No Hit
CCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCA417090.2957345979628921No Hit
ATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACA410150.2908138419872934No Hit
CCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG406470.28820456504346004No Hit
TAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAAT388060.2751510898978156No Hit
CTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGC388050.27514399947133783No Hit
TTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAA377940.267975578302274No Hit
CCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTG335270.23772072852146742No Hit
CTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCT318110.22555355668554897No Hit
GCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGT304120.21563405004309563No Hit
AAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAG259220.18379803515773788No Hit
CGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAA250080.1773173853570214No Hit
GCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCT246070.17447412433942042No Hit
AACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATA244620.17344601250013827No Hit
CTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGA220800.1565566166299997No Hit
TAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGACAG218980.15526615901103866No Hit
CTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGG213980.15172094577213469No Hit
GCTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTG213650.151486961698367No Hit
CCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATT208060.14752341329727237No Hit
GATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTACGAC202340.14346768935196622No Hit
TGCTCTTCCGATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATC195470.13859656636171216No Hit
GCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAG181660.12880468739585935No Hit
CTCCCCATTTCTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGA180210.1277765755565772No Hit
GGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAAC176970.12547927737776743No Hit
CTCTAGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATT176830.12538001140707813No Hit
GACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCA167920.11906244141535122No Hit
ACCGGCATGCCGAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATT159360.11299303635034762No Hit
TAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCT156650.11107153077486165No Hit
GTTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAA156140.11070991902449344No Hit
TATTGACCACACCTCTACCGGCATGCCGAGCAAATGCCTGCAAATCGCTC154890.10982361571476745No Hit
AAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGAAATATTGCTTAC146750.10405200856183179No Hit
GATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGA146430.10382511491454194No Hit
AATTGACGCATGCATTCTTGAAATATTGCTTACGACAGATCGGAAGAGCG145050.10284663606060442No Hit
GAGCAAATGCCTGCAAATCGCTCCCCATTTCTCTAGATTAACCCTAGAAA142890.10131510394139791No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)